All Male Tilapia Fingerlings with Greater Yield and Uniformity

Summary

Tilapia is one of the most important farmed fish and is historically among the first breeds cultivated by humans. The group belongs to the Cichlid species that originated in Africa. Tilapia is easy to farm in different systems ranging from low-tech earthen ponds to intensive high-tech tanks and cages. Some of the limitations in producing tilapia are its small size, slow growth rate and frequent reproduction in captivity. This is because large amounts of metabolic energy go toward reproduction in gestating female tilapia, their growth rate is slowed down and the conversion of feed to flesh is reduced. Male tilapias are bigger and more attractive for production because more of their metabolic energy is channeled toward weight gain, which increases their growth rate and conversion of feed to flesh. Males grow faster and larger, they are therefore more profitable for producers and more acceptable to consumers. The mono-sex male technology was introduced by WorldFish and has rapidly gained popularity with small-scale producers in Africa. The GIFT technology is based on selective breeding processes where brood parents are carefully monitored to obtain improved performance for certain traits that are important for production and marketing. These genetic gains are cumulative and permanent, unlike for other breeding methods with short-lived advantages.

About the Solution

Mixed sex tilapia culturing is technically easy but is associated with lower yields and smaller and non-uniform harvests. Mono-sex culture is more complex, but it is possible to produce all male tilapia fingerlings — as high as 98%. A mono sex, all male tilapia stock is obtained through manual sex selection, hormonal alteration or YY natural male technology (NMT). Genetically improved farmed tilapia (GIFT) transferred from selective breeding programs is ideal for commercial production. In the manual selection, female and male fingerlings are visually separated at the start of the production cycle but this is time-consuming, and nearly half of the stock will be discarded. Alternatively, for commercial production, hormonal alteration of fry is performed by application of the masculine hormone α-Methyltestosterone to the fry feed converting the sex of most fish (98%) from female to male. Research findings show that hormone levels in tilapia fall to normal levels 5 days after the withdrawal of hormone feed. Another approach to sex management relies upon temperature change up to 36o C shortly after hatching, converting the sex of most fish (86%) from female to male, although many of the fry may perish in the process. The end-product of NMT is a complete natural male stock with XY-chromosome, whereas for hormone sex-reversed 50% of the phenotypically males are genetically female with XX-chromosomes.

Culturing mono-sex tilapia is of great importance to aquaculture practice in all African countries because temperatures are highly suitable for fast growth. It offers substantial benefit in terms of its growth and yield per unit area with uniform sizes at harvest. Improved mono-sex tilapia is more resistant to disease, tolerant to adverse weather, and can tolerate broader temperature (12-40°C) and salinity levels (12-15 ppt). Farming of mono-sex tilapia is especially attractive in countries with a strong demand for tilapia such as DR Congo, Ghana, Malawi, Nigeria, Uganda, and Zambia. The process of monosex production using MT hormone is not prohibited, as the European Union and United States markets allow tilapia to be imported from countries producing monosex tilapia. The ministerial decree organizing this issue states that mixing hormone with feed should be done with caution in feed mills.

A large range of tilapia breeds can be used for manual selection, hormonal treatment, YY male technology and GIFT but improved lines are most favorable as they possess traits that further benefit growth rate, feed conversion, size, and hardiness. Crossbreeding of male Blue tilapia (Oreochromis aureus) or Wami tilapia (O. urolepis) and Greenhead tilapia (O. macrochir) with female Nile tilapia (O. nilotica) results in 100% male offspring. Male Blue and Zanzibar tilapia (O. hornorum) that brood with Mozambique tilapia (O. mossambicus) also have exclusively male offspring. Nile tilapia is highly popular due to its faster growth and ability to grow to larger size over a wide range of environmental conditions. Its disease resistance and ability to efficiently utilize very diverse food sources also make it an ideal candidate for culture. A range of sterile transgenic breeds has been developed that can achieve a harvest weight up to 2 kg. Successful farming of mono-sex tilapia requires hatcheries to select and manage brood stock for high quality and quantity of fish seeds. Younger brooders (1 to 1.5 year old) weighing at least 300 g and free of wounds and parasites are needed for establishing a broodstock population with high reproductive efficiency and prolonged life. It is advisable to rejuvenate the broodstock every three years or at the age of 4 to 5 years old.

Normally, brooders are stocked into the spawning units (tanks or hapas) about 15 days before the spawning season. Keep males and females in separate tanks or hapas for feeding before spawning. Feeding starts as on the day following stocking. The water temperature should be raised gradually up to 26°C. After confirming that females are ready to ovulate, both sexes are stocked into the spawning units at a rate of 2 females to 1 male. The average weight of broodstock in each spawning unit must be kept almost equal. Female brood stock must be regularly checked for eggs. If they carry eggs in their mouths, collect the eggs and transfer into the hatching jars, where the incubation period is completed until hatching occurs. After 10-12 days (according to temperature) have elapsed, the newly hatched fish, also called “fry”, can be moved from brooder basins to rearing units. The hormonal mono-sex technology uses a rate of 60 mg artificial testosterone per kilogram of feed. Fry have to be continuously fed with the hormonal agent for 21 days to ensure sexual inversion to a male rate of 98%. After treatment, there should be a few fry under 14 mm in size. However, if more than 5% of the fry are 13 mm or smaller, remove the fry because 25% of them are likely to be females. In the GIFT method, full-sibling families of fish are reared in small, separate enclosures after which they are tagged to track growth against siblings and other individuals.

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Commercialization

Commercially available

Startup Requirements
Fish farmers need to consider the following steps when starting mono-sex tilapia production: 1) Selecting a good location with access to water that is free of pollutants, 2) Availability of matured and fecund brood stock, 3) Installation of aerators for oxygen supply, and 4) Provision of hapa-style nets to raise the fingerlings.
Production Costs
The use of manual sexing and hormonal treatment for obtaining all male stocks are less expensive over the short term but investment must be made during each production cycle. Advanced breeding of the YY male and GIFT methods attract greater startup costs but the maintenance of stocks over the long-term is less. In Kenya mono-sex fingerlings of one month are sold at US $0.1 per individual, whereas mixed-sex stock is 20% cheaper. At a stocking rate of 1,000 fish per cubic meter of water, the cost for farmers is US $100. Fingerlings of mono-sex tilapia for starter stocks are sold at US $0.05 in Nigeria and Zambia. All male fingerlings stocked in earthen ponds or cages can reach 300-900 g in 5 to 8 months of culture, leading to greater profits assuming that feed is accessed or produced in an efficient manner.
Customer Segmentation
Markets for mono-sex tilapia are diverse, including other hatcheries and producers at small or commercial scales.
Potential Profitability
Genetically male tilapia stocks that are bred through the YY technology offer a 2.5-fold greater net return over mixed-sex tilapia and a 90% greater net return compared to hormonally sex-reversed tilapia. Larger sized tilapia may be exported as whole fish or fish fillets. Improved strains of tilapia reach harvestable size faster, and more than 30% increase in harvest volume has been found with GIFT breeds compared to previously used “improved” tilapia breeds, suggesting steady improvement with time. Enhancing the access of fish farmers to improved mono-sex tilapia seed is a viable opportunity that will raise the profitability of hatcheries and African fish farmers alike.
Licensing Requirements
Some of the constituent products and materials needed for GIFT and YY male technology are protected under commercial patent.
Innovation as Public Good
Management of GIFT and YY male tilapia broodstock is offered as a Regional Public Good by WorldFish.

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Hapa Nets for Mass Fingerling Hatchery Production

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